TY  - JOUR
T1  - PUrification and characterization of rubella virus hemagglutinins
AU  - Magnusson P
Y1  - 1969/07/01
N1  - 10.1001/archpedi.1969.02100040096016
JO  - American Journal of Diseases of Children
SP  - 94
EP  - 00
VL  - 118
IS  - 1
N2  - THE hemagglutinating activity of untreated rubella virus material is mainly carried by complete virus particles.1-3 These have a size of 40 to 60 mμ2,4 and a sedimentation constant of 240 S.1,5A subunit hemagglutinin, obtained after treatment with polysorbate 80 (Tween 80) and ether, has been characterized by sedimentation analysis.2,5 The average sedimentation constant of this hemagglutinin was 100 S. The particles varied in size with an average diameter of 25 mμ as estimated from filtration experiments.2The purpose of the present study was to find alternative disintegration methods for rubella virus and to further characterize the subunit hemagglutinins.Materials and Methods 
    Virus Strain.—A strain of rubella virus isolated by Dr. Harvey Liebhaber, Yale University, New Haven, Conn, was used.Tissue Cultures.—VERO cells were propagated in roller bottles with a growth medium consisting of Eagle's basal medium supplied with 10% calf serum.Infection
SN  - 0002-922X
M3  - doi: 10.1001/archpedi.1969.02100040096016
UR  - http://dx.doi.org/10.1001/archpedi.1969.02100040096016
ER  -