RT Journal
A1 Andraweera PH, Dekker GA, Thompson SD, et al
T1 ASsociation of vascular endothelial growth factor +936 c/t single-nucleotide polymorphism with pregnancies complicated by small-for-gestational-age babies
JF Archives of Pediatrics & Adolescent Medicine
JO Archives of Pediatrics & Adolescent Medicine
YR 2011
FD December 1
VO 165
IS 12
SP 1123
OP 1130
DO 10.1001/archpediatrics.2011.796
UL http://dx.doi.org/10.1001/archpediatrics.2011.796
AB Objectives 
                  To examine whether single-nucleotide polymorphisms (SNPs) in VEGFA (−2578 C/A and +936 C/T) associate with small-for-gestational-age (SGA) pregnancies and to identify their effects on first-trimester placental VEGFA expression.Design 
                  Multicenter prospective cohort study.Settings 
                  Adelaide, Australia, and Auckland, New Zealand.Participants 
                  A total of 3234 nulliparous pregnant women, their partners, and their infants.Main Outcome Measures 
                  The SNPs in the parent-infant trios and first-trimester placentae (n = 74) were genotyped. Placental VEGFA messenger RNA expression was determined by quantitative reverse transcription–polymerase chain reaction. Small for gestational age was defined as a birth weight less than the 10th customized birth weight percentile adjusted for maternal height, weight, parity, and ethnicity and for gestational age at delivery and infant sex. Uterine and umbilical artery Doppler was performed at 20 weeks' gestation, and resistance indices greater than the 90th percentile were considered abnormal.Results 
                  Of 2123 pregnancies, 1176 (55.4%) were uncomplicated and 216 (10.2%) had SGA infants. Neonatal VEGFA +936 C/T SNP associates with SGA (adjusted odds ratio [aOR], 1.6; 95% CI, 1.0-2.3), SGA with abnormal Doppler findings (aOR, 3.5; 95% CI, 1.8-7.1), lower birth weight (P = .006), customized birth weight percentile (P = .049), and abnormal uterine artery Doppler findings (OR, 2.5; 95% CI, 1.2-5.4). Maternal VEGFA +936 C/T associates with abnormal umbilical artery Doppler findings (OR, 1.5; 95% CI, 1.1-2.2). VEGFA +936CT+TT first-trimester placentae have 36% lower VEGFA messenger RNA expression compared with CC (P = .045).Conclusion 
                  Neonatal VEGFA +936 C/T associates with SGA, and the association is stronger for SGA with abnormal uterine or umbilical artery Doppler findings. The SNP also associates with reduced first-trimester placental VEGFA expression, suggesting that it may have a role in the pathogenesis of SGA.Trial Registration 
                  clinicaltrials.gov Identifier: ACTRN12607000551493.