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Bilirubin Subfractions

Am J Dis Child. 1988;142(7):700. doi:10.1001/archpedi.1988.02150070014006.
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Sir.—Rosenthal1 has accurately summarized the inadequacies present in many of the non—high-performance liquid chromatography (HPLC) methods for bilirubin analysis. Although we agree that HPLC avoids many of these difficulties and permits the accurate determination of bilirubin subfractions, we think he was remiss in not mentioning that the Ektachem (Eastman Kodak Co, New York) dry-slide method also overcomes many of these problems and gives an accurate quantitation of bilirubin fractions that is equivalent to those obtained by HPLC.2 The Ektachem method, which, according to the manufacturer, is currently available in over 1000 sites and 48% of pediatric hospitals, offers enormous advantages over HPLC in ease of sample analysis. We agree that pediatricians should use the additional information provided by methods that accurately quantitate bilirubin subfractions.3-5


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