Purification of Hepatitis Associated Antigen by Homologous Immunoadsorbents

The purification of the hepatitis associated antigen (HAA) is a time consuming task when classical chromatographic methods are used and immunochemical criteria for purity are applied. We report our experiences with copolymerized immunoadsorbents, as originally described by Avrameas and Terneynck¹ and successfully applied to the purification of homologous antibodies directed against HAA.²

Methods  Initially, 75 ml of human serum containing precipitating antibodies directed against HAA were copolymerized at pH 5.0 using 6.7 ml protein polymer (Chlorethylformiate)¹ and packed in a specially devised filter system³ on a 10μ membrane filter. The copolymerized immunoadsorb- 0.10 u) m 80 E 0.06 0.04 0.02 92 100108 116 124 132 140 148 156164 172 180188 196 204 212 220 228 236 244 252 260 268 276 284 292 300 Fig 1.—On gel filtration (Sephadex G 200 superfine) hepatitis associated antigen (HAA) was demonstrated in peaks 2 and 3 by gel