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Purification of Hepatitis Associated Antigen by Homologous Immunoadsorbents

Wolfgang J. Rapp, MD; H. Erich Lehmann, MD; Rolf Sanwald, MD
Am J Dis Child. 1972;123(4):324-326. doi:10.1001/archpedi.1972.02110100056023.
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The purification of the hepatitis associated antigen (HAA) is a time consuming task when classical chromatographic methods are used and immunochemical criteria for purity are applied. We report our experiences with copolymerized immunoadsorbents, as originally described by Avrameas and Terneynck1 and successfully applied to the purification of homologous antibodies directed against HAA.2

Methods  Initially, 75 ml of human serum containing precipitating antibodies directed against HAA were copolymerized at pH 5.0 using 6.7 ml protein polymer (Chlorethylformiate)1 and packed in a specially devised filter system3 on a 10μ membrane filter. The copolymerized immunoadsorb- 0.10 u) m 80 E 0.06 0.04 0.02 92 100108 116 124 132 140 148 156164 172 180188 196 204 212 220 228 236 244 252 260 268 276 284 292 300 Fig 1.—On gel filtration (Sephadex G 200 superfine) hepatitis associated antigen (HAA) was demonstrated in peaks 2 and 3 by gel


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