SERIAL determinations of antibiotic activity in the serum of infants have been limited by the relatively large amounts of serum required by the tube-dilution method of Rammelkamp.1 The micromethod to be described is a modification of the Rammelkamp technique but is more rapid and requires only 0.025 ml of serum per test. This method makes use of a microtitration system similar to that described by Sever2 for serological investigations of viral infections. The results of its application to a study of the levels of penicillin G after single intramuscular doses given to infants 4-hours to 1-month old will then be presented.
Materials and Method
A Micromethod for Assay of Penicillin in Serum of Infants.Materials:
Capillary tubes for collecting serum
Screw cap vials