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Levels of Penicillin in Serum of Newborn Infants

Am J Dis Child. 1966;111(3):267-271. doi:10.1001/archpedi.1966.02090060077006.
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SERIAL determinations of antibiotic activity in the serum of infants have been limited by the relatively large amounts of serum required by the tube-dilution method of Rammelkamp.1 The micromethod to be described is a modification of the Rammelkamp technique but is more rapid and requires only 0.025 ml of serum per test. This method makes use of a microtitration system similar to that described by Sever2 for serological investigations of viral infections. The results of its application to a study of the levels of penicillin G after single intramuscular doses given to infants 4-hours to 1-month old will then be presented.

Materials and Method  A Micromethod for Assay of Penicillin in Serum of Infants.Materials:

  1. Microtitration system.

    • Dilution plates

    • Wire loops

    • Pipette droppers

    • Capillary tubes and bulbs

    • Blotters

    • Plastic tape

  2. Capillary tubes for collecting serum

  3. Screw cap vials


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