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Filtration of Urine for Quantitation of Cells and Casts

Am J Dis Child. 1964;108(1):19-27. doi:10.1001/archpedi.1964.02090010021003.
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Introduction  Urinary sediment has been recognized for a long time as a useful parameter for the evaluation of health and disease. It is the purpose of this communication to describe and evaluate a simple quantitative method of examination which also permits careful and repeated study of the exfoliated cells. The method consists of the filtration of a measured amount of urine through a membrane of known surface area and of pore size such that all formed elements in the specimen are retained on the surface of the membrane. The formed elements are fixed and stained on this filter membrane which subsequently is rendered transparent. The cells and casts are examined by means of a light microscope and counted. The results are expressed as the number of formed elements per milliliter of urine.

Materials and Method  The following are needed: a side arm filter flask, a Pyrex microanalysis filter holder,* gridded


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