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Laboratory Studies of Influenza Virus Type B Infections in Children

HENRY G. CRAMBLETT, MD; HUGH L. MOFFET, MD; WINSTON-SALEM, NC
Am J Dis Child. 1963;105(6):549-553. doi:10.1001/archpedi.1963.02080040551004.
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An outbreak of influenza virus Type B infections in children residing in The Children's Home, Inc., Winston-Salem, NC, occurred in January, 1962. Virologic studies of these infections permitted the evaluation of the effectiveness of monkey kidney cell cultures for the isolation of influenza B viruses. The influence of the serum content of the maintenance medium upon the recovery of influenza B viruses could be evaluated, since all specimens were inoculated into two tubes of rhesus kidney cultures, one of which was maintained with monkey kidney medium B (2% calf serum)1 and the other with medium 199 (serum-free).2 Specimens for isolation of virus and sera collected during the acute and convalescent phases of illness were available from all children admitted to the infirmary, so that a comparison of serologic and virologic techniques for the laboratory diagnosis of influenza B infections was possible. Epidemiologic and clinical data relating to this

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