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Practical Serum Lipid Determination in Juvenile Diabetes

Am J Dis Child. 1963;105(5):436-438. doi:10.1001/archpedi.1963.02080040438002.
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The usefulness of lipid data in the long-term follow-up of diabetic patients has recently been stressed by observation that diabetes mellitus is a multifaceted disturbance of intermediary metabolism of which the carbohydrate portion is only the most obvious element.1

Although information exists on lipoprotein and cholesterol values of infants 2,3 and children,3,4 the direct application of lipoprotein measurements to the average pediatric practice has not been practical due to the cumbersome nature of ultracentrifugation and electrophoresis which are the physical bases for separating serum lipids into numerous fractions. Lipoprotein precipitation with polyanions requires the use of a spectrophotometer. However, an immunochemical β-lipoprotein determination has been evolved which utilizes an antiserum * specific for β-lipoprotein.5-8 Since it is performed on serum derived from capillary puncture, low-density or β-lipoprotein values of children can be obtained as a routine outpatient procedure.

This paper reports the utilization of this method in the


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