To the Editor: We read with great interest the article by S. Kaplan et al. on "Amine Buffers in the Management of Acidosis" and the accompanying editorial by S. A. Kaplan in the same issue (103:1 [Jan.] 1962).
Some points referred to in each of these articles prompt our comments. In S. Kaplan's article he suggests that protein binding of salicylates limits its excretion. We have investigated binding of salicylate to serum protein and could demonstrate no action of Tris buffer in disrupting this bond (reported to the Central Society for Pediatric Research, October, 1961). We have also investigated the effect of Tris buffer on the binding of bilirubin in an artificially prepared serum and find that the buffer easily dislodges bilirubin from its binding site.
Titration of this material to a pH of 7.4 overcomes in the rat the sloughing effect of this material when injected subcutaneously. The titrated