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April 1960, Vol 99, No. 4 >
Article | April 1960

Diagnosis of Pertussis by Fluorescent Antibody Staining of Nasopharyngeal Smears FREE

PAUL DONALDSON, Ph.D.; JO ANNE WHITAKER, M.D.
[+] Author Affiliations

Submitted for publication July 21, 1959.

5323 Harry Hines Boulevard (19).

Supported in part by Damon Runyon Grant 460(T) and Wyeth Laboratories, Inc.


AMA Am J Dis Child. 1960;99(4):423-427. doi:10.1001/archpedi.1960.02070030425004.
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References
Comments

A reliable and rapid method to establish the diagnosis of pertussis in its early stages is needed to avoid unnecessary exposure of others and to initiate appropriate treatment to reduce the severity and complications of this disease.1 Few laboratories attempt isolation and identification of the etiologic agent because of lack of success with current cultural methods. The fluorescent antibody method for the demonstration of Bordetella pertussis, if reliable, would solve at least some of the clinical and laboratory problems of the disease.

The utility of fluorescent antibody staining for the laboratory diagnosis of bacterial diseases has been established by numerous studies and gives promise of becoming a valuable tool in clinical laboratory diagnosis. Group A streptococci have been observed in direct smears and smears of early enrichment cultures of pharyngeal specimens.2,3 Enteropathogenic Escherichia coli,4 Pasteurella pestis,5 and gonococci,6 for example, have been identified by immunofluorescent

REFERENCES

1 +
Felton, H. M.:  Pertussis, Current Status of Prevention and Treatment , Pediat. Clin. North America , (February) , 1957;, pp. 271-283.
2 +
Halperen, S.; Donaldson, P., and Sulkin, S. E.:  Identification of Streptococci in Bacterial Mixtures and Clinical Specimens with Fluorescent Antibody , J. Bact. 76:223-224, 1958;.
3 +
Moody, M. D.; Ellis, E. C., and Updyke, E. L.:  Staining Bacterial Smears with Fluorescent Antibody: IV. Grouping Streptococci with Fluorescent Antibody , J. Bact. 75:553-560, 1958;.
4 +
Whitaker, J. A.; Page, R. H.; Stulberg, C. S., and Zuelzer, W. W.:  Rapid Identification of Enteropathogenic Escherchia Coli O127:B8 by the Fluorescent Antibody Technique , A.M.A.J. Dis. Child. 95:1-8, 1958;.
5 +
Winter, C. C., and Moody, M. D.:  Rapid Identification of Pasteurella Pestis with Fluorescent Antibody: II. Specific Identification of Pasteurella Pestis in Dried Smears , J. Infect. Dis. 104:281-287, 1959;.
6 +
Deacon, W. E.; Peacock, W. L.; Freeman, E. M., and Harris, A.:  Identification of Neisseria Gonorrhoeae by Means of Fluorescent Antibodies , Proc. Soc. Exper. Biol. & Med. 101:322-325, 1959;.
7 +
de Repentigny, J., and Frappier, A.:  Studies of H. Pertussis Liquid Cultures: III. Localization of Surface Antigens by Means of Fluorescent Antibody , Canad. J. Microbiol. 2:677-683, 1956;.
8 +
Coons, A. H.:  Fluorescent Antibody Methods , Gen. Cytochem. Meth. 1:399-422, 1958;.
9 +
Riggs, J. L.; Siewald, R. J.; Burckhalter, J. H.; Downs, C. M., and Metcalf, T. J.:  Isothiocyanate Compounds as Fluorescent Labeling Agents for Immune Serum , Am. J. Path. 34:1081-1097, 1958;.
10 +
Marshall, J. D.; Eveland, W. C., and Smith, C. W.:  Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic with a Modification of Its Application , Proc. Soc. Exper. Biol. & Med. 98:898-900, 1958;.

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Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

References

1 +
Felton, H. M.:  Pertussis, Current Status of Prevention and Treatment , Pediat. Clin. North America , (February) , 1957;, pp. 271-283.
2 +
Halperen, S.; Donaldson, P., and Sulkin, S. E.:  Identification of Streptococci in Bacterial Mixtures and Clinical Specimens with Fluorescent Antibody , J. Bact. 76:223-224, 1958;.
3 +
Moody, M. D.; Ellis, E. C., and Updyke, E. L.:  Staining Bacterial Smears with Fluorescent Antibody: IV. Grouping Streptococci with Fluorescent Antibody , J. Bact. 75:553-560, 1958;.
4 +
Whitaker, J. A.; Page, R. H.; Stulberg, C. S., and Zuelzer, W. W.:  Rapid Identification of Enteropathogenic Escherchia Coli O127:B8 by the Fluorescent Antibody Technique , A.M.A.J. Dis. Child. 95:1-8, 1958;.
5 +
Winter, C. C., and Moody, M. D.:  Rapid Identification of Pasteurella Pestis with Fluorescent Antibody: II. Specific Identification of Pasteurella Pestis in Dried Smears , J. Infect. Dis. 104:281-287, 1959;.
6 +
Deacon, W. E.; Peacock, W. L.; Freeman, E. M., and Harris, A.:  Identification of Neisseria Gonorrhoeae by Means of Fluorescent Antibodies , Proc. Soc. Exper. Biol. & Med. 101:322-325, 1959;.
7 +
de Repentigny, J., and Frappier, A.:  Studies of H. Pertussis Liquid Cultures: III. Localization of Surface Antigens by Means of Fluorescent Antibody , Canad. J. Microbiol. 2:677-683, 1956;.
8 +
Coons, A. H.:  Fluorescent Antibody Methods , Gen. Cytochem. Meth. 1:399-422, 1958;.
9 +
Riggs, J. L.; Siewald, R. J.; Burckhalter, J. H.; Downs, C. M., and Metcalf, T. J.:  Isothiocyanate Compounds as Fluorescent Labeling Agents for Immune Serum , Am. J. Path. 34:1081-1097, 1958;.
10 +
Marshall, J. D.; Eveland, W. C., and Smith, C. W.:  Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic with a Modification of Its Application , Proc. Soc. Exper. Biol. & Med. 98:898-900, 1958;.

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