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February 1960, Vol 99, No. 2 >
Article | February 1960

Rapid Identification of Candida Albicans by Filamentation on Serum and Serum Substitutes FREE

CLAIRE L. TASCHDJIAN, B.Sc, M.S.Ed.; JAMES J. BURCHALL, M.A.; PHILIP J. KOZINN, M.D.
[+] Author Affiliations

Submitted for publication July 27, 1959.

This study was supported by U.S. Public Health Service Grant E-1751, and by grants from the Squibb Institute for Medical Research and the Jewish Philanthropic League of Brooklyn.


AMA Am J Dis Child. 1960;99(2):212-215. doi:10.1001/archpedi.1960.02070030214011.
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It was noted by Hill and Gebhardt (1956), as well as by Young (1958), that Candida albicans will begin to form mycelia within 60 minutes after subcutaneous injection into mice. Hill and Gebhardt demonstrated that this phenomenon occurs only with C. albicans and C. stellatoidea, while other Candida species fail to form mycelia and are rapidly destroyed in the tissues.

Johnson (1954) observed that C. albicans forms mycelia on human serum but does not mention the time interval of beginning filamentation.

We noted that C. albicans spores produce short germ-tubes within 90 minutes on human serum at 37 to 42 C. This occurred on pooled and individual, hemolyzed and nonhemolyzed, and inactivated sera and on fresh as well as on deep-frozen stored material.

Of other Candida species, only C. stellatoidea, whose species status is doubtful, produced these characteristic short germtubes. C. tropicalis showed long mycelia, carried over with the inoculum

REFERENCES

1 +
Benham, R. W.:  Species of Candida Most Frequently Isolated from Man: Methods and Criteria for Their Identification , J. Chron. Dis. 5:460, 1957;.
2 +
Conant, N. F., et al.: Manual of Clinical Mycology , Ed. 2, Philadelphia, W. B. Saunders Company, 1954;.
3 +
Hill, D. W., and Gebhardt, L. P.:  Morphological Transformation of Candida Albicans in Tissues of Mice , Proc. Soc. Exper. Biol. & Med. 92:640, 1956;.
4 +
Gordon, M. A.:  Differentiation of Yeasts by Means of Fluorescent Antibody , Proc. Soc. Exper. Biol. & Med. 97:694, 1958;.
5 +
Johnson, S. A.:  Candida (Monilia) Albicans: Effects of Amino Acids, Glucose, pH, Chlortetracycline (Aureomycin), Dibasic Sodium and Calcium Phosphates, and Anaerobic and Aerobic Conditions on Its Growth , A.M.A. Arch. Dermat. & Syph. 70:49, 1954;.
6 +
Pagano, J.; Levin, J. D., and Trejo, W.:  Diagnostic Medium for Differentiation of Species of Candida , Antibiotics Ann. 6:137, 1958;.
7 +
Taschdjian, C. L.:  Routine Identification of Candida Albicans: Current Methods and a New Medium , Mycologia 49:332, 1957;.
8 +
Young, G.:  The Process of Invasion and the Persistence of Candida Albicans Injected Intraperitoneally into Mice , J. Infect. Dis. 102:114, 1958;.

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References

1 +
Benham, R. W.:  Species of Candida Most Frequently Isolated from Man: Methods and Criteria for Their Identification , J. Chron. Dis. 5:460, 1957;.
2 +
Conant, N. F., et al.: Manual of Clinical Mycology , Ed. 2, Philadelphia, W. B. Saunders Company, 1954;.
3 +
Hill, D. W., and Gebhardt, L. P.:  Morphological Transformation of Candida Albicans in Tissues of Mice , Proc. Soc. Exper. Biol. & Med. 92:640, 1956;.
4 +
Gordon, M. A.:  Differentiation of Yeasts by Means of Fluorescent Antibody , Proc. Soc. Exper. Biol. & Med. 97:694, 1958;.
5 +
Johnson, S. A.:  Candida (Monilia) Albicans: Effects of Amino Acids, Glucose, pH, Chlortetracycline (Aureomycin), Dibasic Sodium and Calcium Phosphates, and Anaerobic and Aerobic Conditions on Its Growth , A.M.A. Arch. Dermat. & Syph. 70:49, 1954;.
6 +
Pagano, J.; Levin, J. D., and Trejo, W.:  Diagnostic Medium for Differentiation of Species of Candida , Antibiotics Ann. 6:137, 1958;.
7 +
Taschdjian, C. L.:  Routine Identification of Candida Albicans: Current Methods and a New Medium , Mycologia 49:332, 1957;.
8 +
Young, G.:  The Process of Invasion and the Persistence of Candida Albicans Injected Intraperitoneally into Mice , J. Infect. Dis. 102:114, 1958;.

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