Splenectomy in a patient suffering from active rheumatic fever was followed within minutes after manipulation of the spleen by signs of fulminating rheumatic carditis. This led to a study of the effects of splenic extracts as related to rheumatic disease. Two fractions, splenin A, and splenin B, were reported to have antagonistic effects; the former decreased, while the latter increased, capillary permeability. The present book is a preliminary report of a therapeutic trial of splenin A in forty-seven acutely ill patients, in whom the disease varied in severity prior to treatment.
Manufacturing Problems, both in laboratory and bulk quantities, did not permit sufficient material to be produced to evaluate the efficacy of this product. Methods for isolation of the pure substance have not yet been developed. In fact, the potency of the material used in the investigation appeared to be low, and the assay procedure required simplification.
Unfortunately, the evaluation