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AMA Am J Dis Child. 1954;88(5):582-595. doi:10.1001/archpedi.1954.02050100584004.
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AS MENTIONED in the introduction, the purpose of these investigations has been to obtain quantitative data concerning hemoglobin synthesis and hemoglobin loss in premature infants and to evaluate the significance of these factors in the pathogenesis of the anemia of prematurity. Necessary prerequisites for this study were the estimations of the total blood volume and of the proportions of fetal and adult hemoglobins in premature infants. These were reported in the first two parts of this article. With the data now at hand it becomes possible to evaluate the changes in total body hemoglobin and in its component parts without implicating the many variables influencing the hemoglobin concentration in grams per 100 ml. of blood.

METHOD OF APPROACH  The infants studied consisted of 32 prematurely born infants whose birth weights were 2,100 gm. or less. On each infant, samples of blood were taken by heel puncture in the first 24


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