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Article |

Epidemiology and Molecular Identification of Salmonella Infections in Children FREE

Gordon E. Schutze, MD; Russell S. Kirby, PhD; Ellie L. Flick, MSEd; Rossina Stefanova, PhD; Kathleen D. Eisenach, PhD; M. Donald Cave, PhD
[+] Author Affiliations

From the Department of Pediatrics and Pathology, University of Arkansas for Medical Sciences, Arkansas Children's Hospital, Little Rock. Dr Kirby is now with the Department of Obstetrics and Gynecology, University of Wisconsin–Madison Medical School, Sinai Samaritan Medical, Milwaukee, Wis.


Arch Pediatr Adolesc Med. 1998;152(7):659-664. doi:10.1001/archpedi.152.7.659.
Text Size: A A A
Published online

Objective  To explore the role of foods and the environment in the development of infections with Salmonella in infants and children.

Design  Case-controlled survey and the use of pulsed-field gel electrophoresis to establish DNA fingerprint patterns.

Setting  Ambulatory and hospitalized patients at a children's hospital.

Patients or Other Participants  A consecutive sample of children younger than 4 years old who were infected with Salmonella and 3 age-matched controls per patient were to be surveyed. Of the 103 eligible cases of salmonellosis, 90 cases and 264 controls were included in the study.

Data Analysis  Univariate analysis was done using the Mantel-Haenszel χ2 test or the Fisher exact test. The Bonferroni correction was used for multiple comparisons. DNA fingerprints were inspected for identical banding.

Results  Results demonstrated similar diets between cases and controls with the exception of more potato or macaroni salad or coleslaw consumption in the control group (P<.001). DNA fingerprints of Salmonella newport and Salmonella typhimurium demonstrated that all cases were due to unique isolates except in 5 instances involving 12 patients. Seven of these patients could be connected geographically.

Conclusions  Most of the cases of salmonellosis in children younger than 4 years are of a sporadic nature and the major source of infection remains unidentified. For patients infected with identical isolates of Salmonella, a common food source could not be incriminated with the methods used. Environmental contamination or other sources of Salmonella are suggested by these epidemiological data.

Figures in this Article

EACH YEAR in the United States more than 40000 cases of human salmonellosis are reported to the Centers for Disease Control and Prevention with almost one third of these cases involving children younger than 5 years.1 Previous data from Arkansas have demonstrated that the case rates for these infants and children exceed those of the rest of the nation and that most of these cases are sporadic in nature without an identifiable source of infection.2 Clinicians still tend to emphasize contaminated food or food prepared by infected individuals as the major source of infection for these young patients based on what is understood with adults and salmonellosis. Recent data, however, have demonstrated that conclusions drawn from the investigations of food poisoning outbreaks must be of questionable value in explaining the epidemiology of salmonellosis for infants and young children.3 Investigators have suggested that since the Salmonella bacterium is so common in our environment, virtually all infants and young children are exposed to contamination on a daily basis. It is postulated that this daily contamination from the environment in conjunction with factors that affect host resistance may be the most important determinants of infection. The purpose of this study, therefore, was to explore the role of foods and the environment in the development of infections with Salmonella in infants and children.

Investigators were informed when a child younger than 4 years had Salmonella isolated from any specimen submitted to the clinical microbiology laboratory from the outpatient clinics or emergency department or the inpatient service at Arkansas Children's Hospital, Little Rock, from July 1993 through June 1995. The decision to submit specimens for bacterial cultures was at the discretion of the treating physician. Contact by telephone or in person with a parent or guardian of the child was made within 48 hours after notification of the positive culture findings. After informed consent was obtained, demographic data were collected and a questionnaire was administered concerning parental education and occupation, government assistance (eg, Women-Infants-Children Program participation), medical history of the patient having had salmonellosis, involvement of others in the family with a similar illness, preparation and consumption of foods considered to be high risk, pet ownership and type of pet (reptile vs mammal), smoking in the household, day care attendance, and family income. When applicable, questions concerning formula preparation and/or consumption and breast-feeding were included. Three age-matched patients from the general pediatric clinic who presented for routine medical care other than diarrhea were also interviewed on the same day the case was identified as a control group after informed consent was obtained. The first 3 patients with a matching age who had scheduled appointments to the clinic on the day the case was interviewed were chosen as controls. If they refused, the next available patient meeting entry criteria was interviewed. All interviews of cases and controls were conducted by a single, trained interviewer (E.L.F.).

Salmonella isolates were serogrouped in the clinical microbiology laboratory according to the Kaufman-White scheme and sent to the Arkansas Department of Health for serotyping. The 2 most common serotypes underwent further investigation though the use of DNA restriction fragment analysis using pulsed-field gel electrophoresis (PFGE) to identify identical isolates among like serotypes. DNA fragment patterns were visually assessed and each distinct pattern was assigned a pattern number. Since the isolates being compared were of the same serotype, they were considered to be genetically different if there was greater than 1 band variation in PFGE patterns.4 Epidemiological information obtained from the family of patients infected with identical Salmonella serotypes was reviewed extensively looking for a connection that might indicate a common source of infection.

STATISTICAL ANALYSIS

Data were collected using a questionnaire constructed to work efficiently with Epi-Info (Version 5.0B). All data were double entered and verified. Univariate analyses were conducted using the Mantel-Haenszel χ2 test or the Fisher exact test, whichever was appropriate based on expected cell size. The Bonferroni correction was used for the evaluation of multiple comparisons.

DNA PREPARATION, RESTRICTION ENDONUCLEASE DIGESTION, AND PFGE
DNA Preparation

Salmonella organisms were grown overnight at 37°C in nutrient broth and were sedimented by centrifugation and washed in 100-mmol/L Tris hydrochloride (pH 7.5), 100-mmol/L EDTA, and 150-mmol/L sodium chloride. The pellet was mixed with molten 2% low–melting point agarose gel (In Cert Agarose, FMC BioProducts, Rockland, Me) and the suspension was added to plug molds (Bio-Rad, Hercules, Calif). The agarose gel blocks were incubated in solution containing 6-mmol/L Tris hydrochloride (pH 7.5), 1-mol/L sodium chloride, 100-mmol/L EDTA, 0.5% polyoxyethelene 20 cetyl ether (Brij 58, Sigma, St Louis, Mo), 0.2% sodium deoxycholate, 0.5% sarkosyl, and 1-mg/mL lysozyme for 15 hours at 37°C, followed by deproteination in 0.4-mol/L EDTA (pH 9.3), 1% sarkosyl, and 1-mg/mL proteinase K at 50°C for 24 hours. The cell debris and proteinase K were removed by multiple washes in CHEF-TE (clamped homogeneous electric field in a 0.1-mol/L Tris and 0.1-mol/L EDTA buffer) buffer—100-mmol/L Tris hydrochloride (pH 7.5)—and 100-mmol/L EDTA. DNA plugs were stored at 4°C in CHEF-TE buffer until digestion with a restriction enzyme.

Restriction Enzyme Digestion

Genomic DNA was restricted with I XbaI (5′-TCTAGA-3′), and SpeI (5′-ACTAGT-3′). Isolates with similar fingerprint patterns with XbaI and SpeI underwent subsequent restriction with NheI (5′-GCTAGC-3′), AvrII (5′-CCTAGG-3′), AseI (5′-ATTAAT-3′), or NotI (5′-GCGGCCGC-3′) (New England Bio Labs, Beverly, Mass, and Promega Co, Madison, Wis). The agarose gel blocks were washed for 3 hours at 4°C in TM buffer—100-mmol/L Tris hydrochloride (pH 8) and 5-mmol/L magnesium chloride—followed by equilibration in the appropriate restriction enzyme buffer for 1 hour at 4°C. Fresh buffer containing 5 to 25 units of the chosen enzyme was added to the plugs and they were incubated for 16 hours at 37°C. The enzyme reaction was terminated by adding EDTA (pH 8) to a final 1-mol/L concentration.

Pulsed-Field Gel Electrophoresis

Pulsed-field gel electrophoresis of the digested samples was performed by contour-clamped homogeneous electric field on a CHEF-DR II system (Bio-Rad Laboratories, Richmond, Calif) in 1% agarose gels in 0.5 X TBE buffer—0.1-mol/L Tris, 0.1-mol/L boric acid, 0.2-mmo/L EDTA (pH 8) for 14 or 24 hours at 200 V and 14°C. The ramped-pulse times varied according to the enzyme, ranging from 1 to 60 seconds. The DNA size standards used were a bacteriophage lambda ladder consisting of concatemers starting at 48.5 kilobase pairs (Bio-Rad Laboratories, Richmond, Calif) as well as a midrange II marker with the smallest band at 24 kilobase pairs (New England Bio Labs). The gels were stained with ethidium bromide and photographed on a UV transilluminator.

During the study period, 103 cases of human salmonellosis in eligible patients were identified. Thirteen cases were excluded because contact could not be established in 8, 3 were already included in the study, and 2 refused to participate. Seventy percent of the cases occurred between April and September. The case and control groups did not differ in relation to age, race, sex, or history of chronic diseases (Table 1). During the week prior to the development of the patients' illness there was no difference in the type of medications or the number of illnesses in other family members for cases and controls regardless of age. Specifically, 17% of cases and 14% of controls described another member of the family with diarrhea. For infants younger than 3 months, 29% of cases and 22% of controls (P>.2) had at least 1 other family member with diarrhea. There was also no difference between the groups in regards to pet ownership, smoking in the household, day care attendance, parental education, employment in the poultry industry, and household income of less than $10000. The control patients, however, were more likely to be supported by the Women-Infants-Children Program (73% vs 60%; P<.02) and Medicaid (63% vs 43%; P<.01) than the cases.

Table Graphic Jump LocationTable 1. Demographics and Chronic Diseases of Salmonella Cases and Controls*

Review of the food data demonstrated that the only difference in food consumption noted was that the controls were more likely to have eaten potato or macaroni salad or coleslaw (Table 2). There were also no differences noted between cases and controls for infants still consuming formula (Table 3). Among those patients who did not boil their water prior to mixing the infant's formula, the source of the water was similar for cases and controls. For cases, municipal water was used in 24 (70%) and well or bottled water was used in 5 (15%) while for controls municipal water was used in 52 (81%), bottled water in 9 (14%), and well water in 3 (5%). The difference between cases and controls as to the use of well water was not significant.

Table Graphic Jump LocationTable 2. Food Preparation and Consumption 1 Week Prior to Interview*
Table Graphic Jump LocationTable 3. Eating Habits of Infants Still Consuming Formula*

Salmonella was isolated from the stool in 86 cases, the blood in 3 cases, and the cerebrospinal fluid in 1 case. The most common serotype isolated was Salmonella newport (n=30), followed by Salmonella typhimurium (n=25), Salmonella javiana (n=7), Salmonella heidelberg (n=4), Salmonella ibadan (n=3), and other species (n=21). Evaluating risk factors by the organism isolated revealed only that patients infected with S typhimurium tended to be older (mean age, 20 months; P<.02) as compared with those infected with S newport (mean age, 13 months), S javiana (mean age, 11 months), or other Salmonella species (mean age, 11 months). Specifically, no notable environmental or food exposures were identified when the data were evaluated based on the organism isolated.

Pulsed-field gel electrophoresis was performed on 29 S newport and 22 S typhimurium isolates (Figure 1). Restriction endonuclease analysis patterns of S newport and S typhimurium generated by PFGE after XbaI and SpeI digestion were 12 and 9, respectively, for the S newport isolates and 20 and 18, respectively, for the S typhimurium isolates. Combining XbaI and SpeI patterns for S newport demonstrated 23 distinct combinations of patterns. Nineteen of these combinations represented single patients, while 4 of the identical patterns involved 10 patients. Restriction endonuclease patterns generated with NheI, AvrI, NotI, or AseI were not helpful in further differentiating the isolates. Combining the results of both enzymes for S typhimurium demonstrated 21 distinct combinations, each represented by a single patient except for 1 common fingerprint pattern that was noted among 2 patients. Restriction endonuclease patterns generated with NheI and NotI were not helpful in the further differentiation of S typhimurium isolates.

Place holder to copy figure label and caption
Figure 1.

This gel is a demonstration of the different patterns obtained with pulsed-field gel electrophoresis using XbaI and Salmonella typhimurium. Lane is a marker lane using a 48.5–kilobase pair λ ladder. Visual inspection demonstrates that the patterns in lanes 3, 4, and 8 are identical.

Graphic Jump Location

The demographic data of patients infected with isolates that had similar DNA fingerprint patterns were further reviewed (Figure 2). The average age of the children involved in these clusters was 15.5 months, which was not different from the other 78 cases (13.8 months). All patients except for 2 (1 with S newport, 1 with S typhimurium identified) used city water. None of the children involved had similar diet histories. Of the 4 common clusters involving 10 patients with S newport, 2 clusters involved 5 patients who lived in or adjacent to the same city as another patient with the same isolate although as much as 3 months may have separated the illnesses. In the 1 pair of identical DNA fingerprint patterns involving S typhimurium, the 2 patients were infected 7 months apart and lived in separate cities and counties. The second child, however, had spent the day in the home town of the first child within 72 hours of the beginning of their illnesses. No other common source could be identified.

Place holder to copy figure label and caption
Figure 2.

This gel demonstrates 2 identical DNA fingerprint patterns of Salmonella typhimurium from patients in lanes 2 through 7. Restriction enzymes used included AvrII (lanes 2 and 3), SpeI (lanes 4 and 5), and XbaI (lanes 6 and 7). DNA fingerprint patterns are identical for 2 isolates of Salmonella newport from 2 patients in lanes 9 through 14. Restriction enzymes used included AvrII (lanes 9 and 10), SpeI (lanes 11 and 12), and XbaI (lanes 13 and 14). Lanes 1 and 15 are marker lanes using a 48.5–kilobase pair λ ladder.

Graphic Jump Location

Human epidemics of salmonellosis are commonly attributed to contaminated foods, but the role of foods in the development of sporadic disease in infants and children is not understood. Data generated in this study in infants and young children would suggest that the dietary habits prior to the development of infection between cases and controls were remarkably similar. Recent data have suggested that contaminated eggs remain important vehicles for the transmission of Salmonella in adults even in the absence of recognized outbreaks.5 Such data for young children are not supported by the current study because of the lack of egg consumption by our patients, the lack of infections due to Salmonella enteritidis, and the fact that Arkansas has not had a problem with egg contamination.6

Risk factors for infants who do not consume table foods have identified the use of iron-fortified formulas and the lack of breast-feeding as major risk factors for the development of disease.7,8 Although questions about the iron content of formulas were not added until the second year of the survey, no differences were noted between groups in the use of such formulas. The numbers of infants breast-feeding or using any breast milk in their diet in this study were too few to demonstrate the protective effect that has been previously described in our patient population.8 Although there was no statistical difference noted between the groups concerning well water usage, the use of contaminated well water still might have a role in the development of disease in a subgroup of patients. Freshwater contamination from the effluent of the poultry processing plants is usually suggested by local physicians as a major cause for cases of salmonellosis in Arkansas. Salmonella typhimurium has been associated with contaminated poultry and both S newport and S typhimurium can be recovered from contaminated freshwater,9,10 but previous ecological analyses demonstrated no correlation between the presence of poultry processing plants or hatcheries and the rate of human salmonellosis in Arkansas.2 The role of contaminated water in the development of salmonellosis, however, still remains questionable.

OTHER RECOGNIZED risk factors for the development of salmonellosis such as chronic diseases or lower socioeconomic class were not found to predominate in our cases as compared with our controls. The socioeconomic status of our patients and controls was similar. The fact that more control children were supported by Medicaid and the Women-Infants-Children Program was probably because of the fact that the controls were patients who received their routine medical care in our general pediatric clinic where an emphasis on enrolling eligible patients into these programs exists. When evaluating our patients and controls by absolute income there was no difference noted between the 2 groups. Specifically, there was no difference between the 2 groups regarding yearly incomes of less than $10000. An attempt to identify environmental or food risk factors based on the organisms isolated also was unsuccessful. Salmonella newport and S typhimurium have been demonstrated in previous investigations to be the 2 most common isolates in Arkansas.2 The discovery that S typhimurium was more commonly found to infect the older child is of interest, but at present, its meaning is unclear.

Analysis of chromosomal DNA restriction patterns by PFGE was chosen for our study since it is widely available and has been proven to demonstrate genetic variations among like serotypes of Salmonella.1115 In the current study, enough diversity was observed among 44 (86%) of the fingerprint patterns that it appears the use of XbaI and SpeI are useful epidemiological tools for S newport and S typhimurium. With the use of these restriction enzymes, the 2 most common serotypes were found to have 44 different fingerprint combinations. This would indicate that most of the isolates were not clonally related, which is consistent with the conclusion that most of the cases are sporadic in nature. Further evaluation of isolates with identical patterns using other enzymes was not found to aid in differentiating the serotypes in the 5 clusters of patients with similar patterns. Because of the diversity of the DNA fingerprint patterns obtained with the use of these restriction enzymes, the appearance of identical patterns suggests either the presence of a common contaminating source of infection or that there is limited sequence divergence in isolates of these particular serotypes.

Our epidemiological data, however, support the former. Seven of the children had a history of living in or traveling to the same town as others with identical isolates. Because these infants and children had different diet histories, the source of their infection was more likely from their immediate environment and not directly from contaminated foods. Past studies have demonstrated that cultures taken from the households of infected infants and children have demonstrated that the dirt from around the home, the local playground, and samples from vacuum cleaners or broom straws from the home have been found to harbor Salmonella.16,17 Since infants and children are very inquisitive, are orally fixated, and are constantly exploring their environment, this type of contamination would be of great importance but would not explain how patients could be infected with identical organisms separated by long distances and time.

An infected person contaminating the child's environment, however, could explain the issues with time and distance. The median duration of excretion of Salmonella from all infected individuals is approximately 5 weeks and although 90% of adults stop excreting organisms after 9 weeks and only 1% are excreting at 1 year, 60% of children younger than 5 years are excreting after 20 weeks and approximately 5% still excrete the organism at 1 year after the development of their illness.18 In previous bacteriologic investigations of household contacts of infants and young children with salmonellosis, it has been noted that approximately one third of household contacts are shedding Salmonella with approximately 50% being asymptomatic.19,20 Infants and young children are more likely to have extended or more intimate periods of handling by their caretakers than older children, thus increasing their risk of exposure to infection if the caretaker is excreting Salmonella. Our data, however, demonstrated that an equal number of patient and control families had other individuals in the home with diarrhea. This was also true even among the patients younger than 3 months, an age that has been associated with intrafamilial spread.20 Unfortunately, we had no method for detecting asymptomatic infections among family members; the importance of such an individual cannot be overlooked.

In conclusion, this study demonstrates the complexities of identifying the source of human salmonellosis in infants and children. For patients infected with identical isolates of Salmonella, a common contaminating food source was not identified with the methods used but environmental contamination or other sources of Salmonella might account for these infections. For patients with unique isolates, a major source of infection remains unidentified. Further evaluations of these patients to include bacterial evaluations of their homes, contacts, and foods would be required to adequately support these findings. Based on these data, however, when clinicians encounter patients with human salmonellosis who are younger than 4 years, time spent questioning the family about the environment surrounding the patient rather than a dietary history may deliver more useful information.

Accepted for publication February 23, 1998.

This research was sponsored by prime grant 93-34211-8360, subcontract UA AES 93-102 from the Food Safety Consortium under the US Department of Agriculture, issued by the University of Arkansas, Agricultural Experimental Station, Fayetteville.

Presented as an abstract at the 94th American Society for Microbiology General Meeting, Las Vegas, Nev, May 27, 1994.

The authors thank Kelly J. Kelleher, MD, MPH, University of Pittsburgh Medical Center, Pittsburgh, Pa, for his helpful comments and critical review in the presentation of the statistical results.

Editor's Note: My Italian ethnic background helps me to understand the significance of the study group consuming less macaroni salad, but coleslaw?—Catherine D. DeAngelis, MD

Reprints: Gordon E. Schutze, MD, Arkansas Children's Hospital, 800 Marshall St, Little Rock, AR 72202-3591 (e-mail: schutzegordone@exchange.uams.edu).

Centers for Disease Control and Prevention, Summary of notifiable diseases, United States 1994. MMWR Morb Mortal Wkly Rep. 1994;431- 80
Schutze  GEFlick  ELPope  SKLofgren  JPKirby  RS Epidemiology of salmonellosis in Arkansas. South Med J. 1995;88195- 199
Link to Article
Haddock  RL The origins of infant salmonellosis. Am J Public Health. 1993;83772
Link to Article
Tenover  FCArbeit  RDGoering  RV  et al.  Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995;332233- 2239
Hedberg  CWDavid  MJWhite  KEMacDonald  KLOsterholm  MT Role of egg consumption in sporadic Salmonella enteritidis and Salmonella typhimurium infections in Minnesota. J Infect Dis. 1993;167107- 111
Link to Article
Schutze  GEFawcett  HALewno  MJFlick  ELKirby  RS Prevalence of Salmonella enteritidis in poultry shell eggs in Arkansas. South Med J. 1996;89889- 891
Link to Article
Haddock  RLCousens  SNGuzman  CC Infant diet and salmonellosis. Am J Public Health. 1991;81997- 1000
Link to Article
France  GLMarmer  DJSteele  RW Breast-feeding and Salmonella infection. AJDC. 1980;134147- 152
Burton  GA  JrGunnison  DLanza  GR Survival of pathogenic bacteria in various freshwater sediments. Appl Environ Microbiol. 1987;53633- 638
Angulo  FJTippen  SSharp  DJ  et al.  A community waterborne outbreak of salmonellosis and the effectiveness of a boil water order. Am J Public Health. 1997;87580- 584
Link to Article
Thong  KLCheong  YMPuthucheary  SKoh  CLPang  T Epidemiological analysis of sporadic Salmonella typhi isolates and those from outbreaks by pulsed-field gel electrophoresis. J Clin Microbiol. 1994;321135- 1141
Nair  SPoh  CLLim  YSTay  LGoy  KT Genome fingerprinting of Salmonella typhi by pulsed-field gel electrophoresis for subtyping common phage types. Epidemiol Infect. 1994;113391- 402
Link to Article
Thong  KLPuthucheary  SYassin  RM  et al.  Analysis of Salmonella typhi isolates from Southeast Asia by pulsed-field gel electrophoresis. J Clin Microbiol. 1995;331938- 1941
Baquar  NBurnens  AStanley  J Comparative evaluation of molecular typing of strains from a national epidemic due to Salmonella brandenburg by rRNA gene and IS200 probes and pulsed-field gel electrophoresis. J Clin Microbiol. 1994;321876- 1880
Dietrich  SAltamirano  JVDownes  FPMartin  R Molecular epidemiology of a multi-state outbreak of Salmonella poona.  Abstracts Of The 94Th General Meeting Of The American Society For Microbiology May 26, 1994 Las VegasNev. Abstract C-421
Haddock  RLMalilay  J A search for infant salmonellosis risk factors on Guam. Southeast Asian J Trop Med Public Health. 1986;1738- 42
Haddock  RL Salmonella in vacuum cleaners [letter]. Lancet. 1986;11637
Link to Article
Buchwald  DSBlaser  MJ A review of human salmonellosis, II: duration of excretion following infection with nontyphi SalmonellaRev Infect Dis. 1984;6345- 356
Link to Article
Rosenstein  BJ Salmonellosis in infants and children: epidemiologic and therapeutic considerations. J Pediatr. 1967;701- 7
Link to Article
Wilson  RFeldman  RADavis  JLaVenture  M Salmonellosis in infants: the importance of intrafamilial transmission. Pediatrics. 1982;69436- 438

Figures

Place holder to copy figure label and caption
Figure 1.

This gel is a demonstration of the different patterns obtained with pulsed-field gel electrophoresis using XbaI and Salmonella typhimurium. Lane is a marker lane using a 48.5–kilobase pair λ ladder. Visual inspection demonstrates that the patterns in lanes 3, 4, and 8 are identical.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.

This gel demonstrates 2 identical DNA fingerprint patterns of Salmonella typhimurium from patients in lanes 2 through 7. Restriction enzymes used included AvrII (lanes 2 and 3), SpeI (lanes 4 and 5), and XbaI (lanes 6 and 7). DNA fingerprint patterns are identical for 2 isolates of Salmonella newport from 2 patients in lanes 9 through 14. Restriction enzymes used included AvrII (lanes 9 and 10), SpeI (lanes 11 and 12), and XbaI (lanes 13 and 14). Lanes 1 and 15 are marker lanes using a 48.5–kilobase pair λ ladder.

Graphic Jump Location

Tables

Table Graphic Jump LocationTable 1. Demographics and Chronic Diseases of Salmonella Cases and Controls*
Table Graphic Jump LocationTable 2. Food Preparation and Consumption 1 Week Prior to Interview*
Table Graphic Jump LocationTable 3. Eating Habits of Infants Still Consuming Formula*

References

Centers for Disease Control and Prevention, Summary of notifiable diseases, United States 1994. MMWR Morb Mortal Wkly Rep. 1994;431- 80
Schutze  GEFlick  ELPope  SKLofgren  JPKirby  RS Epidemiology of salmonellosis in Arkansas. South Med J. 1995;88195- 199
Link to Article
Haddock  RL The origins of infant salmonellosis. Am J Public Health. 1993;83772
Link to Article
Tenover  FCArbeit  RDGoering  RV  et al.  Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995;332233- 2239
Hedberg  CWDavid  MJWhite  KEMacDonald  KLOsterholm  MT Role of egg consumption in sporadic Salmonella enteritidis and Salmonella typhimurium infections in Minnesota. J Infect Dis. 1993;167107- 111
Link to Article
Schutze  GEFawcett  HALewno  MJFlick  ELKirby  RS Prevalence of Salmonella enteritidis in poultry shell eggs in Arkansas. South Med J. 1996;89889- 891
Link to Article
Haddock  RLCousens  SNGuzman  CC Infant diet and salmonellosis. Am J Public Health. 1991;81997- 1000
Link to Article
France  GLMarmer  DJSteele  RW Breast-feeding and Salmonella infection. AJDC. 1980;134147- 152
Burton  GA  JrGunnison  DLanza  GR Survival of pathogenic bacteria in various freshwater sediments. Appl Environ Microbiol. 1987;53633- 638
Angulo  FJTippen  SSharp  DJ  et al.  A community waterborne outbreak of salmonellosis and the effectiveness of a boil water order. Am J Public Health. 1997;87580- 584
Link to Article
Thong  KLCheong  YMPuthucheary  SKoh  CLPang  T Epidemiological analysis of sporadic Salmonella typhi isolates and those from outbreaks by pulsed-field gel electrophoresis. J Clin Microbiol. 1994;321135- 1141
Nair  SPoh  CLLim  YSTay  LGoy  KT Genome fingerprinting of Salmonella typhi by pulsed-field gel electrophoresis for subtyping common phage types. Epidemiol Infect. 1994;113391- 402
Link to Article
Thong  KLPuthucheary  SYassin  RM  et al.  Analysis of Salmonella typhi isolates from Southeast Asia by pulsed-field gel electrophoresis. J Clin Microbiol. 1995;331938- 1941
Baquar  NBurnens  AStanley  J Comparative evaluation of molecular typing of strains from a national epidemic due to Salmonella brandenburg by rRNA gene and IS200 probes and pulsed-field gel electrophoresis. J Clin Microbiol. 1994;321876- 1880
Dietrich  SAltamirano  JVDownes  FPMartin  R Molecular epidemiology of a multi-state outbreak of Salmonella poona.  Abstracts Of The 94Th General Meeting Of The American Society For Microbiology May 26, 1994 Las VegasNev. Abstract C-421
Haddock  RLMalilay  J A search for infant salmonellosis risk factors on Guam. Southeast Asian J Trop Med Public Health. 1986;1738- 42
Haddock  RL Salmonella in vacuum cleaners [letter]. Lancet. 1986;11637
Link to Article
Buchwald  DSBlaser  MJ A review of human salmonellosis, II: duration of excretion following infection with nontyphi SalmonellaRev Infect Dis. 1984;6345- 356
Link to Article
Rosenstein  BJ Salmonellosis in infants and children: epidemiologic and therapeutic considerations. J Pediatr. 1967;701- 7
Link to Article
Wilson  RFeldman  RADavis  JLaVenture  M Salmonellosis in infants: the importance of intrafamilial transmission. Pediatrics. 1982;69436- 438

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