0
We're unable to sign you in at this time. Please try again in a few minutes.
Retry
We were able to sign you in, but your subscription(s) could not be found. Please try again in a few minutes.
Retry
There may be a problem with your account. Please contact the AMA Service Center to resolve this issue.
Contact the AMA Service Center:
Telephone: 1 (800) 262-2350 or 1 (312) 670-7827  *   Email: subscriptions@jamanetwork.com
Error Message ......
Article |

Helicobacter pylori and Infantile Colic FREE

Abdelrazak Mansour Ali, MD
[+] Author Affiliations

Author Affiliation: Department of Pediatrics, Al-Azhar University, Cairo, Egypt.


Arch Pediatr Adolesc Med. 2012;166(7):648-650. doi:10.1001/archpediatrics.2011.1241.
Text Size: A A A
Published online

Objective To determine whether Helicobacter pylori is associated with infantile colic.

Design Case-control study.

Settings Local tertiary hospital in rural Gizan, Saudi Arabia.

Participants A total of 55 patients with infantile colic who were 2 weeks to 4 months of age and who fulfilled modified Wessel criteria (ie, crying and fussy behavior) and a total of 30 healthy controls with no history of colic who were matched by country of origin, age, sex, and ethnicity to the 55 colicky infants.

Main Outcome Measure Helicobacter pylori infection determined by H pylori stool antigen testing.

Results Of the 55 patients presenting with infantile colic, 45 (81.8%) tested positive for H pylori ; of the 30 healthy controls, 7 (23.3%) tested positive for H pylori (odds ratio, 15.3 [95% CI, 17.9-29.8]).

Conclusion H pylori infection is associated with infantile colic and may be a causative factor.

Since the discovery of Helicobacter pylori by Marshall and Warren1 in 1983, a new era of discovery and understanding of gastroduodenal pathology has begun. H pylori infection is a common bacterial infection in humans, and the organism is the most prevalent gastric microbial pathogen. The outcome of chronic H pylori infection varies from asymptomatic gastritis to peptic ulcerations to gastric malignancies.2 It is currently estimated that half of the world's human population is infected with the gastric pathogen H pylori. However, the prevalence of H pylori is not homogenous worldwide; it varies and is dependent on the patient's age, country of origin, ethnic background, and socioeconomic conditions during childhood.3 The virulence factors of H pylori and the host genetic factors are both considered important determinants of disease.4 In an attempt to search for the pathogenesis of some infantile disorders that have an unclear etiology, we investigated the potential role of H pylori infection in infantile colic. The cause remains enigmatic, despite its long history and its relatively frequent occurrence. Its prevalence ranged from 5% to 40% depending on the definition and methods used.57 Possible explanations were suggested: painful gut contractions, lactose intolerance, social factors,8,9 or high levels of motilin hormone secreted by the small intestinal cells.10 Colic is similar in both breast-fed and bottle-fed infants. The prevalence of colic among exclusively breast-fed infants, among exclusively formula-fed infants, and among both breast-fed and formula-fed infants was 22%, 20%, and 18%, respectively. There was no significant relationship between the source of early infant nutrition and the development of colic.9 Infantile colic has no clear treatment, and its management varies among physicians.11

Approval of the present study was received from the administration of Alemeis National Hospital in Sabia, Saudi Arabia. Cases were recruited from the local tertiary hospital in rural Gizan, Saudi Arabia, from May to September 2009. Cases admitted for routine outpatient clinical care, for vaccination, or for medical services were selected. A questionnaire was administered to the mothers of infants 2 weeks to 4 months of age and included questions about maternal health behaviors, demographic characteristics, and the current source of infant nutrition (whether the infant was exclusively breast fed, exclusively formula fed, or fed both breast milk and formula). Standardized measures (the 6-item state scale of the Spielberger State–Trait Anxiety Inventory, the Support Behaviors Inventory, and Anticipatory Guidance) that assessed maternal anxiety, postnatal depression, and social support were incorporated into the first questionnaire because these constructs have been suspected to play a role in the development of colic.9 Mothers were also asked to complete a short questionnaire based on the Ames cry score, which is composed of 3 questions, each with its own 4 response categories that are scored from 0 to 3. This short questionnaire asks about the frequency and the average and maximum duration of an infant's cries during the past week. Overall scores, calculated by summing the scores of individual items, range from 0 to 9, with a score of 3 or greater indicating colic.12

Ninety-two participants were categorized into 2 groups (ie, case and control groups), and allocations were concealed from the participants' parents until after pretesting was complete. Of the 92 participants, 85 completed the study. There were no statistically significant differences in baseline sociodemographics between the 7 participants who dropped out of the study and the 85 participants who completed the study. There were 55 case infants aged 2 weeks to 4 months who fulfilled modified Wessel criteria for infantile colic, which criteria meant that a well, thriving infant cried for 3 hours daily for more than 3 days every week for more than 3 weeks,4,1214 excluding other causes of crying. The results of a detailed examination of the infant were recorded to rule out any underlying causes of excessive crying (eg, central nervous system abnormalities, infections, trauma, foreign body in the eye, fractured bone, or other gastrointestinal dysfunctions).4 Eligible controls were selected from the same population and matched to case infants by country of origin, age, sex, and race. The study controls were identified as having no colic or no history of colic, and no severe distressing illness or abnormalities.

The case and control groups were investigated for H pylori using a stool antigen test. This one-step test is a chromatographic immunoassay for the qualitative detection of H pylori infections (Alcon Laboratories Inc). It is a relatively simple, reliable, more applicable, and noninvasive test of H pylori infections in children.15,16Helicobacter pylori fecal antigen has shown a high degree of sensitivity, specificity, and positive and negative predictive values.16

All analyses were performed using SPSS (SPSS Inc). The demographic characteristics of cases and controls were compared using the Fisher exact test, and odds ratios and 95% CIs were calculated.

There were 28 boys and 27 girls enrolled in the case group and 17 boys and 13 girls enrolled in the control group. The 2 groups were similar in terms of age, sex, race, prematurity, insurance status, maternal education, and maternal depression (Table). Of the 55 case infants, 45 (81.8%) tested positive for H pylori, and 10 (18.2%) tested negative. Of the 30 controls, only 7 (23.3%) tested positive for H pylori, and 23 (76.7%) tested negative (odds ratio, 15.3 [95% CI, 7.9-29.8]).

Table Graphic Jump LocationTable. Demographic Characteristics of 85 Participants With or Without Infantile Colica

Numerous studies have elucidated the pathogenesis, immunology, and H pylori gastrointestinal–related disorders (in addition to the extraintestinal manifestations) associated with H pylori infection, but, to my knowledge, none of these studies have investigated the role of H pylori in infantile colic. The present study found a strong association between H pylori infection and infantile colic.

Other studies have suggested that this association might be causal. Recently, strains of lactic acid bacteria, selected for their capability of promoting the production of interleukin 10 (IL-10) and, consequently, the proliferation of CD4+CD25+ T-cell receptors have been used for the treatment of infantile colic.1719 A recent study by Savino et al20 has found that a daily dose of a probiotic helps improve colic symptoms. The more recent research conducted by Zhou et al21 demonstrated the role of lactobacilli in treating H pylori –related diseases, and the results indicated that viable lactobacilli prevented the development of H pylori Sydney strain 1 (SS1) lipopolysaccharide (LPS)–activated Toll-like receptor 4 (TLR4) pathways in SGC-7901 cells, leading to the inhibitory effects of lactobacilli on IL-8 production stimulated by H pylori SS1 LPSs.21

Some studies have demonstrated that H pylori stimulates the release of IL-8 from gastric epithelia, which initiates inflammatory damage to gastric mucosa as the basis of the pathogenesis of H pylori infections. H pylori LPS is the major initiator in H pylori –induced IL-8 production. Considering the novel finding that H pylori is an indigenous biota in gastric microflora, including lactobacilli, and the hypothesis that the disturbance of the microecosystem plays a more important role in the pathogenesis of H pylori, the restoration of the gastric microecosystem by the therapeutic effects of lactobacilli on H pylori –associated diseases has been demonstrated.22

Christensen et al22 suggested that different species of lactobacillus exert very different dendritic cell activation patterns, and at least one species may be capable of inhibiting the activities of other species. Thus, there is potential for TH1/TH2/TH3-driving capacities of the gut dendritic cell to be modulated according to the composition of gut microflora. Moreover, Kao et al23 suggested that dendritic cells participate in the host immune response against H pylori and that their suppression by H pylori –secreted factors may explain why infected hosts fail to prevent bacterial colonization.

In a recent study,21 2 soluble proteins with molecular sizes of 75 and 40 kDa were purified from supernatant of Lactobacillus rhamnosus and named P75 and P40, respectively, which ameliorated apoptosis of intestinal epithelia treated with tumor necrosis factor, interferon γ, or IL-1α and promoted cell growth. Similarly, it could intervene in H pylori SS1-LPS–activated TLR4 signaling through modulating other pathways in SGC-7901 cells.21 In short, the interaction between the host immune factors and the H pylori virulence factors determines the outcome of H pylori infection (ie, the development of infant colic after the second week of life). Gradually, the microecosystem and other gastrointestinal flora did come into action to dominate and modulate (H pylori vs host) immune responses in order to drive the interactive pathways toward the relief of colic symptoms after 3 to 4 months of age.

I did not find any significant difference between severity of infantile colic and concentration of H pylori stool antigen that was compatible with the findings in Madani et al24 and Bahú et al25; both of those studies24,25 found no correlation between severe gastritis and marked bacterial colonization. Helicobacter pylori infections may be considered the etiologic pathogenic organism of infantile colic.

Correspondence: Abdelrazak Mansour Ali, MD, Department Pediatrics, Al-Azhar University, 9 Mahmoud Ali St, Faisal Giza, Cairo, Egypt (abdelrazak_ali@yahoo.com).

Accepted for Publication: October 10, 2011.

Financial Disclosure: None reported.

Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration.  Lancet. 1984;1(8390):1311-1315
PubMed   |  Link to Article
Kato S, Sherman PM. What is new related to Helicobacter pylori infection in children and teenagers?  Arch Pediatr Adolesc Med. 2005;159(5):415-421
PubMed   |  Link to Article
Sherman PM. Appropriate strategies for testing and treating Helicobacter pylori in children: when and how?  Am J Med. 2004;117:(suppl 5A)  30S-35S
PubMed
Roberts DM, Ostapchuk O, O’Brien JG. Infantile colic.  Am Fam Physician. 2004;70(4):735-740, 741-742
PubMed
Rautava P, Lethtonen L, Helenius H, Sillanpää M. infantile coli: child and family three years later.  Pediatics. 1995;96(1, pt 1):43-47
PubMed
Räihä H, Lehtonen L, Korhonen T, Korvenranta H. Family life 1 year after infantile colic.  Arch Pediatr Adolesc Med. 1996;150(10):1032-1036
PubMed   |  Link to Article
Canivet C, Jakobsson I, Hagander B. Infant colic: follow-up at 4 years old age: still more “emotional”.  Acta Paediatr. 2000;89(1):13-17
PubMed   |  Link to Article
Wade S, Kilgour T. Extracts from “clinical evidence”: infantile colic [published correction appears in BMJ. 2001;323(7314):674].  BMJ. 2001;323(7310):437-440
PubMed   |  Link to Article
Abdel Razak MM, Samir MZ. Infantile colic: is there an association with the source of early infant nutrition?  Zagazig Univ Med J. 2002;8(7):1233-1242
Knott L.Infantile colic. Patient.co.uk website. http://www.patient.co.uk/doctor/Infantile-Colic.htm. Updated May 9, 2009. Accessed March 21, 2012
Cohen-Silver J, Ratanapalan S. Management of infantile colic. A Review.  Clin Pediatr (Phila). 2009;48(1):14-17
PubMed   |  Link to Article
Wessel MA, Cobb JC, Jackson EB, Harris GS Jr, Detwiler AC. Paroxysmal fussing in infancy, sometimes called colic.  Pediatrics. 1954;14(5):421-435
PubMed
Canadian Paediatric Society.  Dietary manipulations for infantile colic.  Paediatr Child Health. 2003;8(7):449-452
PubMed
Leung AK. Infantile colic.  Am Fam Physician. 1987;36(3):153-156
PubMed
Gulcan EM, Varol A, Kutlu T,  et al.  Helicobacter pylori stool antigen test.  Indian J Pediatr. 2005;72(8):675-678
PubMed   |  Link to Article
Sabbi T, De Angelis P, Colistro F, Dall’Oglio L, di Abriola GF, Castro M. Efficacy of noninvasive tests in the diagnosis of Helicobacter pylori infection in pediatric patients.  Arch Pediatr Adolesc Med. 2005;159(3):238-241
PubMed   |  Link to Article
Smits HH, Engering A, van der Kleij D,  et al.  Selective probiotic bacteria induce IL-10-producing regulatory T cells in vitro by modulating dendritic cell function through dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin.  J Allergy Clin Immunol. 2005;115(6):1260-1267
PubMed   |  Link to Article
Valeur N, Engel P, Carbajal N, Connolly E, Ladefoged K. Colonization and immunomodulation by Lactobacillus reuteri ATCC 55730 in the human gastrointestinal tract.  Appl Environ Microbiol. 2004;70(2):1176-1181
PubMed   |  Link to Article
Pessi T, Sütas Y, Hurme M, Isolauri E. Interleukin-10 generation in atopic children following oral Lactobacillus rhamnosus GG.  Clin Exp Allergy. 2000;30(12):1804-1808
PubMed   |  Link to Article
Savino F, Pelle E, Palumeri E, Oggero R, Miniero R. Lactobacillus reuteri (American Type Culture Collection Strain 55730) versus simethicone in the treatment of infantile colic: a prospective randomized study.  Pediatrics. 2007;119(1):e124-e130
PubMed   |  Link to Article
Zhou C, Ma FZ, Deng XJ, Yuan H, Ma HS. Lactobacilli inhibit interleukin-8 production induced by Helicobacter pylori lipopolysaccharide-activated Toll-like receptor 4.  World J Gastroenterol. 2008;14(32):5090-5095
PubMed   |  Link to Article
Christensen HR, Frøkiaer H, Pestka JJ. Lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic cells.  J Immunol. 2002;168(1):171-178
PubMed
Kao JY, Rathinavelu S, Eaton KA,  et al.  Helicobacter pylori -secreted factors inhibit dendritic cell lL-12 secretion: a mechanism of ineffective host defense.  Am J Physiol Gastrointest Liver Physiol. 2006;291(1):G73-G81
PubMed   |  Link to Article
Madani S, Rabah R, Tolia V. Diagnosis of Helicobacter pylori infection from antral biopsies in pediatric patients: is urease test that reliable?  Dig Dis Sci. 2000;45(6):1233-1237
PubMed   |  Link to Article
Bahú MdGS, da Silveira TR, Maguilnick I, Ulbrich-Kulczynski J. Endoscopic nodular gastritis: an endoscopic indicator of high-grade bacterial colonization and severe gastritis in children with Helicobacter pylori J Pediatr Gastroenterol Nutr. 2003;36(2):217-222
PubMed   |  Link to Article

Figures

Tables

Table Graphic Jump LocationTable. Demographic Characteristics of 85 Participants With or Without Infantile Colica

References

Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration.  Lancet. 1984;1(8390):1311-1315
PubMed   |  Link to Article
Kato S, Sherman PM. What is new related to Helicobacter pylori infection in children and teenagers?  Arch Pediatr Adolesc Med. 2005;159(5):415-421
PubMed   |  Link to Article
Sherman PM. Appropriate strategies for testing and treating Helicobacter pylori in children: when and how?  Am J Med. 2004;117:(suppl 5A)  30S-35S
PubMed
Roberts DM, Ostapchuk O, O’Brien JG. Infantile colic.  Am Fam Physician. 2004;70(4):735-740, 741-742
PubMed
Rautava P, Lethtonen L, Helenius H, Sillanpää M. infantile coli: child and family three years later.  Pediatics. 1995;96(1, pt 1):43-47
PubMed
Räihä H, Lehtonen L, Korhonen T, Korvenranta H. Family life 1 year after infantile colic.  Arch Pediatr Adolesc Med. 1996;150(10):1032-1036
PubMed   |  Link to Article
Canivet C, Jakobsson I, Hagander B. Infant colic: follow-up at 4 years old age: still more “emotional”.  Acta Paediatr. 2000;89(1):13-17
PubMed   |  Link to Article
Wade S, Kilgour T. Extracts from “clinical evidence”: infantile colic [published correction appears in BMJ. 2001;323(7314):674].  BMJ. 2001;323(7310):437-440
PubMed   |  Link to Article
Abdel Razak MM, Samir MZ. Infantile colic: is there an association with the source of early infant nutrition?  Zagazig Univ Med J. 2002;8(7):1233-1242
Knott L.Infantile colic. Patient.co.uk website. http://www.patient.co.uk/doctor/Infantile-Colic.htm. Updated May 9, 2009. Accessed March 21, 2012
Cohen-Silver J, Ratanapalan S. Management of infantile colic. A Review.  Clin Pediatr (Phila). 2009;48(1):14-17
PubMed   |  Link to Article
Wessel MA, Cobb JC, Jackson EB, Harris GS Jr, Detwiler AC. Paroxysmal fussing in infancy, sometimes called colic.  Pediatrics. 1954;14(5):421-435
PubMed
Canadian Paediatric Society.  Dietary manipulations for infantile colic.  Paediatr Child Health. 2003;8(7):449-452
PubMed
Leung AK. Infantile colic.  Am Fam Physician. 1987;36(3):153-156
PubMed
Gulcan EM, Varol A, Kutlu T,  et al.  Helicobacter pylori stool antigen test.  Indian J Pediatr. 2005;72(8):675-678
PubMed   |  Link to Article
Sabbi T, De Angelis P, Colistro F, Dall’Oglio L, di Abriola GF, Castro M. Efficacy of noninvasive tests in the diagnosis of Helicobacter pylori infection in pediatric patients.  Arch Pediatr Adolesc Med. 2005;159(3):238-241
PubMed   |  Link to Article
Smits HH, Engering A, van der Kleij D,  et al.  Selective probiotic bacteria induce IL-10-producing regulatory T cells in vitro by modulating dendritic cell function through dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin.  J Allergy Clin Immunol. 2005;115(6):1260-1267
PubMed   |  Link to Article
Valeur N, Engel P, Carbajal N, Connolly E, Ladefoged K. Colonization and immunomodulation by Lactobacillus reuteri ATCC 55730 in the human gastrointestinal tract.  Appl Environ Microbiol. 2004;70(2):1176-1181
PubMed   |  Link to Article
Pessi T, Sütas Y, Hurme M, Isolauri E. Interleukin-10 generation in atopic children following oral Lactobacillus rhamnosus GG.  Clin Exp Allergy. 2000;30(12):1804-1808
PubMed   |  Link to Article
Savino F, Pelle E, Palumeri E, Oggero R, Miniero R. Lactobacillus reuteri (American Type Culture Collection Strain 55730) versus simethicone in the treatment of infantile colic: a prospective randomized study.  Pediatrics. 2007;119(1):e124-e130
PubMed   |  Link to Article
Zhou C, Ma FZ, Deng XJ, Yuan H, Ma HS. Lactobacilli inhibit interleukin-8 production induced by Helicobacter pylori lipopolysaccharide-activated Toll-like receptor 4.  World J Gastroenterol. 2008;14(32):5090-5095
PubMed   |  Link to Article
Christensen HR, Frøkiaer H, Pestka JJ. Lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic cells.  J Immunol. 2002;168(1):171-178
PubMed
Kao JY, Rathinavelu S, Eaton KA,  et al.  Helicobacter pylori -secreted factors inhibit dendritic cell lL-12 secretion: a mechanism of ineffective host defense.  Am J Physiol Gastrointest Liver Physiol. 2006;291(1):G73-G81
PubMed   |  Link to Article
Madani S, Rabah R, Tolia V. Diagnosis of Helicobacter pylori infection from antral biopsies in pediatric patients: is urease test that reliable?  Dig Dis Sci. 2000;45(6):1233-1237
PubMed   |  Link to Article
Bahú MdGS, da Silveira TR, Maguilnick I, Ulbrich-Kulczynski J. Endoscopic nodular gastritis: an endoscopic indicator of high-grade bacterial colonization and severe gastritis in children with Helicobacter pylori J Pediatr Gastroenterol Nutr. 2003;36(2):217-222
PubMed   |  Link to Article

Correspondence

CME
Also Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
Please click the checkbox indicating that you have read the full article in order to submit your answers.
Your answers have been saved for later.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
Submit a Comment

Multimedia

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Articles Related By Topic
Related Collections
PubMed Articles
JAMAevidence.com

Users' Guides to the Medical Literature
Clinical Scenario

The Rational Clinical Examination
Peptic ulcer disease and GERD most commonly cause organic upper gastrointestinal symptoms. Peptic...