We're unable to sign you in at this time. Please try again in a few minutes.
We were able to sign you in, but your subscription(s) could not be found. Please try again in a few minutes.
There may be a problem with your account. Please contact the AMA Service Center to resolve this issue.
Contact the AMA Service Center:
Telephone: 1 (800) 262-2350 or 1 (312) 670-7827  *   Email: subscriptions@jamanetwork.com
Error Message ......
Other Articles |


Am J Dis Child. 1950;79(1):59-64. doi:10.1001/archpedi.1950.04040010069007.
Text Size: A A A
Published online

THE PURPOSE of this paper is to establish further the usefulness of hyaluronidase, a relatively new agent in facilitating administration of fluids in pediatric practice, and to present results of numerous studies on toxicity performed to insure the relative safety of alidase,®1 the commercial hyaluronidase product used in this clinical investigation.

Duran-Reynals2 and McClean3 were the first to note a spreading factor, hyaluronidase, present in testicular extract aiding spread of subcutaneously injected dye and fluid. This factor has since been obtained from many sources, including snake venom, leech, spider and bee extract, spermatozoa, some carcinomas and many bacteria. Chain and Duthie4 identified the spreading factor as a mucolytic enzyme which acts on hyaluronic acid,5 a mucopolysaccharide which in its native form is a gel or mucin clot not bound to protein.

By use of histochemical methods such as metachromic reactions of certain dyes, acid mucopolysaccharides


Sign in

Purchase Options

• Buy this article
• Subscribe to the journal

First Page Preview

View Large
First page PDF preview





Also Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
Please click the checkbox indicating that you have read the full article in order to submit your answers.
Your answers have been saved for later.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
Submit a Comment


Some tools below are only available to our subscribers or users with an online account.

Sign in

Purchase Options

• Buy this article
• Subscribe to the journal

Related Content

Customize your page view by dragging & repositioning the boxes below.