KLING and associates1 suggested that micro-organisms in sewage—probably of the genus Bodo—might be carriers of poliomyelitis virus. Recently, Evans and Osterud2 reported that protozoa from pond, lake and river water failed to yield a significant increase of poliomyelitis virus when several strains of the virus were used. Six strains of Bodo, two of Monas and one each of Pleuromonas, Oikomonas, Tetrahymena and Uronema derived from sewage failed to support the growth of poliomyelitis virus to an extent which would be significant with reference to the finding of the virus in sewage.2
In our experiments, we tried to determine whether Amoeba proteus, normally found in fresh water, could be infected, and, if so, whether it could carry the virus of poliomyelitis.
METHODS AND MATERIALS
This experiment was initiated over two years ago with Amoeba proteus obtained from Elon College, N. C. The original material was in spring