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EXCRETION OF 17-KETO STEROIDS BY NORMAL AND BY ABNORMAL CHILDREN

NATHAN B. TALBOT, M.D.; ALLAN M. BUTLER, M.D.; R. A. BERMAN, B.S.; P. M. RODRIGUEZ, B.S.; E. A. MACLACHLAN, B.S.
Am J Dis Child. 1943;65(3):364-375. doi:10.1001/archpedi.1943.02010150012002.
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INTRODUCTION  The present article extends previous observations on the total output of neutral urinary 17-keto steroids of children.1 The establishment of normal standards of excretion of these substances by normal children of different ages and sexes, as determined by the analytic methods now available,2 is essential for the evaluation of the excretion of these substances in the urine of abnormal children. Section I of this paper presents such normal figures as standards of comparison. Observations on children with physiologic and pathologic variations in growth and development are reported in sections II and III.

METHODS AND MATERIAL  Accurately timed overnight or twenty-four hour specimens of urine were collected, and approximately 5 cc. of hydrochloric acid was added as preservative. The urine was stored at ice box temperature until it was assayed. After hydrolysis, extraction and partial purification, the total content of neutral 17-keto steroids of each sample was determined

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