In reading the literature on cholesterol metabolism one is confronted with the most contradictory results. To a great extent this confusion is due to two circumstances: (1) the use of different species of animals in the investigations and (2) the inadequacy of the methods used. The latter should no longer be a source of difficulty, because accurate methods for the detection of cholesterol in tissues as well as in blood are now available. Confusion due to the first point can also be eliminated easily if consideration is given to the fact that results obtained for one animal do not necessarily hold true for an animal of a different species.
So far as the cholesterol metabolism of human beings is concerned, only a few essential facts have been established. Gamble and Blackfan1 found consistently negative balances in infants, and they consequently concluded that infants can synthesize cholesterol. This important finding