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Am J Dis Child. 1935;49(3):625-637. doi:10.1001/archpedi.1935.01970030071006.
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Extensive experience has identified old tuberculin as one of the most valuable and dependable diagnostic agents used by physicians. The detection of persons who have been infected with tubercle bacilli is so important, however, from the point of view of the clinic and of public health that refinements of tuberculin designed to enhance its specificity and potency are desirable. Seibert's excellent investigations,1 which resulted in the isolation of the active principle of tuberculin, have made special products available for the Mantoux test, and recently a few reports have appeared in which experiences with the tuberculoprotein precipitated by ammonium sulphate (human tubercle bacillus protein) are recorded. The special object of this paper is to discuss the relative testing merits of three tuberculins, as revealed by my data and those published by other investigators, and also to submit the results of studies of the areas (determined by the area-by-weight method)2


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