Bliss1 has devised a method for the determination of the amide nitrogen of the blood. With this method he reported as the normal for adults between 134 and 144 mg. per hundred cubic centimeters of blood, with an average of 139 mg. He stated that the error in the method is within 1 per cent. As far as we are aware, no values for the normal amide nitrogen of the blood of children have appeared in the literature.
This paper records the results of a study of the amide nitrogen of the blood in fifty children. The specimens of blood were obtained in the morning before breakfast, from the median basilic vein, a tourniquet of light rubber tubing being employed with moderate pressure.
The lactic acid (method of Friedmann, Cotonio and Shaffer2) and the carbon dioxide-combining power (Van Slyke) were also determined on the same specimens, in order