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Am J Dis Child. 1927;34(3):404-407. doi:10.1001/archpedi.1927.04130210079008.
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Originality is not claimed in proposing a microprecipitation test of blood serum and spinal fluid for syphilis. The Kahn1 test is a modification of the Sachs-Georgi reaction,2 an extract prepared according to the method of Neymann and Gager3 and a short period of incubation being used. The basis of most of the precipitation reactions in syphilis is Michaelis'4 observation that diluted alcoholic extract of a syphilitic liver added to heated syphilitic serum produces a precipitation. A review of the literature may be found in Kahn's book1 and in an article by Kline and Young.5

The time-consuming complexity, the difficulty in standardization and the possible sources of error in the complement fixation blood test have stimulated a search for a more reliable and a simpler test. The Wassermann test is an indirect, empiric procedure requiring five different animal reagents for its completion: human serum (antibody),


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