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Removal of Bovine Insulin From Cow's Milk Formula and Early Initiation of Beta-Cell Autoimmunity in the FINDIA Pilot Study

Outi Vaarala, MD, DMSc; Jorma Ilonen, MD, DMSc; Terhi Ruohtula, MA; Jouni Pesola, MD; Suvi M. Virtanen, MD, DMSc; Taina Härkönen, PhD; Matti Koski, MA; Harri Kallioinen, MSc (Tech); Olli Tossavainen, DSc (Tech); Tuija Poussa, MSc; Anna-Liisa Järvenpää, MD, DMSc; Jorma Komulainen, MD, DMSc; Raisa Lounamaa, MD, DMSc; Hans K. Åkerblom, MD, DMSc; Mikael Knip, MD, DMsC
Arch Pediatr Adolesc Med. 2012;166(7):608-614. doi:10.1001/archpediatrics.2011.1559.
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Objective  To test whether weaning to a bovine insulin–free cow's milk formula (CMF) reduces type 1 diabetes mellitus–associated autoantibodies in children at genetic risk.

Design  Randomized, double-blind pilot trial (Finnish Dietary Intervention Trial for the Prevention of Type 1 Diabetes [FINDIA]).

Setting  Three pediatric hospitals in Finland from May 15, 2002, to November 22, 2005.

Participants  A total of 1113 infants with HLA-conferred susceptibility to type 1 diabetes were randomly assigned to receive study infant formulas; 908 children provided at least 1 follow-up blood sample (last follow-up, June 2009).

Intervention  The CMF (n = 389), whey-based hydrolyzed formula (WHF) (n = 350), or whey-based FINDIA formula essentially free of bovine insulin (n = 365) during the first 6 months of life whenever breast milk was not available.

Main Outcome Measures  Primary outcome was beta-cell autoimmunity monitored at ages 3, 6, and 12 months and then annually until age 3 years. Autoantibodies to insulin, the 65-kDa isoform of glutamic acid decarboxylase, and the tyrosine phosphatase–related IA-2 molecule were screened, and islet cell autoantibodies and autoantibodies to zinc transporter 8 were analyzed in infants whose primary screening test results were positive.

Results  In the intention-to-treat analysis, 6.3% of children in the CMF group, 4.9% of those in the WHF group, and 2.6% of children in the FINDIA group were positive for at least 1 autoantibody by age 3 years. The odds ratios were 0.75 (95% CI, 0.37-1.54) in the WHF group and 0.39 (0.17-0.91) in the FINDIA group when compared with the CMF group. In the treatment-received analysis, the corresponding odds ratios were 0.81 (95% CI, 0.37-1.76) and 0.23 (0.08-0.69).

Conclusion  In comparison with ordinary CMF, weaning to an insulin-free CMF reduced the cumulative incidence of autoantibodies by age 3 years in children at genetic risk of type 1 diabetes mellitus.

Trial Registration  clinicaltrials.gov Identifier: NCT01055080

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Figure 1. Participant flow diagram. FINDIA indicates Finnish Dietary Intervention Trial for the Prevention of Type 1 Diabetes; ITT, intention-to-treat; and TR, treatment-received.

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Figure 2. Kaplan-Meier curves for survival without positive autoantibodies according to the treatment-received analysis. Curves represent survival in infants who received cow's milk formula (CMF) (n = 236), whey-based hydrolyzed formula (WHF) (n = 187), or insulin-free whey-based formula (Finnish Dietary Intervention Trial for the Prevention of Type 1 Diabetes [FINDIA]) (n = 229) and in the group not exposed to any study formula (no exposure) (n = 256). The CMF group was included as a reference group.

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